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cytokine levels  (R&D Systems)


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    R&D Systems cytokine levels
    Cytokine Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine levels/product/R&D Systems
    Average 94 stars, based on 166 article reviews
    cytokine levels - by Bioz Stars, 2026-02
    94/100 stars

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    As 2 O 3 affects Treg and Th17 cells differentiation. (A,B) Schematic representative of Th17 and Treg-cell differentiation. (C,D) ELISA of IL-17A and MMP13 in supernatants of Th17 cells polarized, IL-10 and TGF-β release in supernatants of Treg-cell production in the presence or absence of As 2 O 3 (0.5 µM), measured after 24 h of restimulation with anti-CD3/CD28. (E,F) RT-qPCR analysis of the expression RORγt for Th17 cells, of STAT5 in Treg cells. Cycling threshold (CT) values are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are represented as means ± SEM of six independent experiments. # p < 0.05, ## p < 0.01 vs. nonpolarized CD4 + T cells; * p < 0.05, ** p < 0.01 vs. Th17 cells nontreated with As 2 O 3 (one-way ANOVA).

    Journal: Frontiers in Pharmacology

    Article Title: Single Cell RNA-Seq Analysis Identifies Differentially Expressed Genes of Treg Cell in Early Treatment-Naive Rheumatoid Arthritis By Arsenic Trioxide

    doi: 10.3389/fphar.2021.656124

    Figure Lengend Snippet: As 2 O 3 affects Treg and Th17 cells differentiation. (A,B) Schematic representative of Th17 and Treg-cell differentiation. (C,D) ELISA of IL-17A and MMP13 in supernatants of Th17 cells polarized, IL-10 and TGF-β release in supernatants of Treg-cell production in the presence or absence of As 2 O 3 (0.5 µM), measured after 24 h of restimulation with anti-CD3/CD28. (E,F) RT-qPCR analysis of the expression RORγt for Th17 cells, of STAT5 in Treg cells. Cycling threshold (CT) values are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are represented as means ± SEM of six independent experiments. # p < 0.05, ## p < 0.01 vs. nonpolarized CD4 + T cells; * p < 0.05, ** p < 0.01 vs. Th17 cells nontreated with As 2 O 3 (one-way ANOVA).

    Article Snippet: The cytokines IL-17A and IL-10 (D1700 and D1000, respectively, purchased from R&D systems, Minneapolis, United States) and MMP13 (E-EL-H0134c, Elabscience Biotechnology, Wuhan, China) were determined using enzyme-linked immunosorbent assay (ELISA).

    Techniques: Cell Differentiation, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing

    Selected genes linked to inflammation and immunoregulation in As 2 O 3 -treated RA Treg cells relative to control.

    Journal: Frontiers in Pharmacology

    Article Title: Single Cell RNA-Seq Analysis Identifies Differentially Expressed Genes of Treg Cell in Early Treatment-Naive Rheumatoid Arthritis By Arsenic Trioxide

    doi: 10.3389/fphar.2021.656124

    Figure Lengend Snippet: Selected genes linked to inflammation and immunoregulation in As 2 O 3 -treated RA Treg cells relative to control.

    Article Snippet: The cytokines IL-17A and IL-10 (D1700 and D1000, respectively, purchased from R&D systems, Minneapolis, United States) and MMP13 (E-EL-H0134c, Elabscience Biotechnology, Wuhan, China) were determined using enzyme-linked immunosorbent assay (ELISA).

    Techniques: Control